Query_essential_gene_IDSPBC1271.13
Query_essential_gene_namemrpl8
Query_essential_gene_productmitochondrial ribosomal protein subunit L8 (predicted)
Suppressor_gene_IDSPBC16G5.01
Suppressor_gene_namerpn12
Suppressor_gene_product19S proteasome regulatory subunit Rpn12
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D

E

F


G

H

I

J

K

L

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days

C: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_10days

D: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

E: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days

F: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_10days

G: Control | Selection for the OE plasmid (suppressor overexpression ON)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days

I: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_10days

J: Control | Selection for the OE plasmid (suppressor overexpression OFF)

K: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days

L: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_10days