Query_essential_gene_ID | SPBC1271.13 |
Query_essential_gene_name | mrpl8 |
Query_essential_gene_product | mitochondrial ribosomal protein subunit L8 (predicted) |
Suppressor_gene_ID | SPBP19A11.03c |
Suppressor_gene_name | mts4 |
Suppressor_gene_product | 19S proteasome regulatory subunit Mts4 |
Mutagen | C-BOE |
Suppressor_mutation_type | Point mutation at the orginal locus |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | 412:S>F |
A
B
C
D
E
F
A: Candidate | No selection
B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion
C: Control | No selection
D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion
E: Quantification | Illustration of the strain arrangement of the semi-quantification plate
F: Quantification | Quantification in restreaking format