Query_essential_gene_IDSPBC839.08c
Query_essential_gene_nameits8
Query_essential_gene_productpig-N (predicted)
Suppressor_gene_IDSPBC32H8.08c
Suppressor_gene_nameomh5
Suppressor_gene_productalpha-1,2-mannosyltransferase Omh5 (predicted)
MutagenT-BOE
Suppressor_mutation_typeDeletion
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteNA

A

B

C

D

E


F

G

A: Candidate | No selection

B: Candidate | Selection for suppressor gene mutation

C: Candidate | Selection for essential gene deletion

D: Candidate | Selection for essential and suppressor genes double mutation

E: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

F: Quantification | Illustration of the strain arrangement of the semi-quantification plate

G: Quantification | Quantification in restreaking format