Query_essential_gene_ID | SPBC839.08c |
Query_essential_gene_name | its8 |
Query_essential_gene_product | pig-N (predicted) |
Suppressor_gene_ID | SPBC32H8.08c |
Suppressor_gene_name | omh5 |
Suppressor_gene_product | alpha-1,2-mannosyltransferase Omh5 (predicted) |
Mutagen | T-BOE |
Suppressor_mutation_type | Deletion |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | NA |
A
B
C
D
E
F
G
A: Candidate | No selection
B: Candidate | Selection for suppressor gene mutation
C: Candidate | Selection for essential gene deletion
D: Candidate | Selection for essential and suppressor genes double mutation
E: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion
F: Quantification | Illustration of the strain arrangement of the semi-quantification plate
G: Quantification | Quantification in restreaking format