Query_essential_gene_IDSPBC1198.04c
Query_essential_gene_namezas1
Query_essential_gene_producttranscription factor Zas1 (predicted)
Suppressor_gene_IDSPAC19A8.13
Suppressor_gene_nameusp101
Suppressor_gene_productU1 snRNP-associated protein Usp101
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D

E

F

G

H

I

J

K

L

M

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

D: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

E: Control | Selection for the OE plasmid (suppressor overexpression ON)

F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

G: Control | Selection for the OE plasmid (suppressor overexpression OFF)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

I: Quantification | Illustration of the strain arrangement of the semi-quantification plate

J: Quantification | Quantification in restreaking format (suppressor overexpression ON)

K: Quantification | Quantification in restreaking format (suppressor overexpression OFF)

L: Quantification | Quantification in restreaking format (suppressor overexpression ON)_5days

M: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_5days