Query_essential_gene_IDSPBC32H8.10
Query_essential_gene_namecdk9
Query_essential_gene_productP-TEFb-associated cyclin-dependent protein kinase Cdk9
Suppressor_gene_IDSPAC23G3.01
Suppressor_gene_namerpb2
Suppressor_gene_productRNA polymerase II complex subunit Rpb2
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note431:T>I

A

B

C

D

E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format