Query_essential_gene_ID | SPBC32H8.10 |
Query_essential_gene_name | cdk9 |
Query_essential_gene_product | P-TEFb-associated cyclin-dependent protein kinase Cdk9 |
Suppressor_gene_ID | SPAC23C4.19 |
Suppressor_gene_name | spt5 |
Suppressor_gene_product | DSIF transcription elongation factor complex subunit Spt5 |
Mutagen | C-BOE |
Suppressor_mutation_type | Point mutation at the orginal locus |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | 504:S>F |
A
B
C
D
E
F
A: Candidate | No selection
B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion
C: Control | No selection
D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion
E: Quantification | Illustration of the strain arrangement of the semi-quantification plate
F: Quantification | Quantification in restreaking format