Query_essential_gene_IDSPBC11B10.03
Query_essential_gene_namecog8
Query_essential_gene_productGolgi transport complex subunit Cog8 (predicted)
Suppressor_gene_IDSPCC74.01
Suppressor_gene_namesly1
Suppressor_gene_productSNARE binding protein Sly1 (predicted)
MutagenC-BOE
Suppressor_mutation_typeAn ectopic mutant version
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note504:S>F

A

B

C

D

E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format