Query_essential_gene_IDSPBC119.18
Query_essential_gene_namemdm35
Query_essential_gene_productmitochondrial distribution and morphology protein Mdm35 (predicted)
Suppressor_gene_IDSPCC736.08
Suppressor_gene_namecbf11
Suppressor_gene_productCBF1/Su(H)/LAG-1 family transcription factor Cbf11
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D

E

F


G

H

I

J

K

L

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_3days

C: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_8days

D: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

E: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_3days

F: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_8days

G: Control | Selection for the OE plasmid (suppressor overexpression ON)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_3days

I: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_8days

J: Control | Selection for the OE plasmid (suppressor overexpression OFF)

K: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_3days

L: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_8days