Query_essential_gene_ID | SPBC119.18 |
Query_essential_gene_name | mdm35 |
Query_essential_gene_product | mitochondrial distribution and morphology protein Mdm35 (predicted) |
Suppressor_gene_ID | SPCC736.08 |
Suppressor_gene_name | cbf11 |
Suppressor_gene_product | CBF1/Su(H)/LAG-1 family transcription factor Cbf11 |
Mutagen | OP-BOE |
Suppressor_mutation_type | Overexpression-plasmid |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | C-terminally YFH-tagged ORF OE |
A
B
C
D
E
F
G
H
I
J
K
L
A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)
B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_3days
C: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_8days
D: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)
E: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_3days
F: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_8days
G: Control | Selection for the OE plasmid (suppressor overexpression ON)
H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_3days
I: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_8days
J: Control | Selection for the OE plasmid (suppressor overexpression OFF)
K: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_3days
L: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_8days