Query_essential_gene_IDSPBC27B12.01c
Query_essential_gene_namemmm1
Query_essential_gene_productERMES complex subunit Mmm1 (predicted)
Suppressor_gene_IDSPCC1450.16c
Suppressor_gene_nameptl1
Suppressor_gene_producttriacylglycerol lipase ptl1
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D

E

F

G

H

A: Candidate | Quantification in restreaking format (suppressor overexpression ON)

B: Candidate | Quantification in restreaking format (suppressor overexpression OFF)

C: Candidate | Quantification in restreaking format (suppressor overexpression ON)

D: Candidate | Quantification in restreaking format (suppressor overexpression OFF)

E: Control | Selection for the OE plasmid (suppressor overexpression ON)

F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

G: Control | Selection for the OE plasmid (suppressor overexpression OFF)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)