Query_essential_gene_ID | SPBC27B12.01c |
Query_essential_gene_name | mmm1 |
Query_essential_gene_product | ERMES complex subunit Mmm1 (predicted) |
Suppressor_gene_ID | SPAC3F10.18c |
Suppressor_gene_name | rpl4102 |
Suppressor_gene_product | 60S ribosomal protein L41 (predicted) |
Mutagen | OP-BOE |
Suppressor_mutation_type | Overexpression-plasmid |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | Taggless ORF OE |
A
B
C
D
E
F
G
H
A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)
B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)
C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)
D: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)
E: Control | Selection for the OE plasmid (suppressor overexpression ON)
F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)
G: Control | Selection for the OE plasmid (suppressor overexpression OFF)
H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)