Query_essential_gene_IDSPBC409.15
Query_essential_gene_nametsr2
Query_essential_gene_productrRNA processing protein Tsr2 (predicted)
Suppressor_gene_IDSPBC1289.03c
Suppressor_gene_namespi1
Suppressor_gene_productRan GTPase Spi1
MutagenC-BOE
Suppressor_mutation_typeAn ectopic mutant version
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note141:K>I

A

B


C

D


E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format