Query_essential_gene_IDSPBC337.06c
Query_essential_gene_namecwf15
Query_essential_gene_productcomplexed with Cdc5 protein Cwf15
Suppressor_gene_IDSPAC227.08c
Suppressor_gene_nameyth1
Suppressor_gene_productmRNA cleavage and polyadenylation specificity factor complex Yth1
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note97:C>R

A

B

C

D

E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format