Query_essential_gene_IDSPBC409.14c
Query_essential_gene_namemrps17
Query_essential_gene_productmitochondrial ribosomal protein subunit S17 (predicted)
Suppressor_gene_IDSPBC1734.13
Suppressor_gene_nameatp3
Suppressor_gene_productF1-ATPase gamma subunit (predicted)
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note282:R>C

A

B


C

D


E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format