Query_essential_gene_ID | SPBC1709.07 |
Query_essential_gene_name | erg27 |
Query_essential_gene_product | 3-keto sterol reductase Erg27 (predicted) |
Suppressor_gene_ID | SPBC3F6.02c |
Suppressor_gene_name | erg26 |
Suppressor_gene_product | 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase Erg26 (predicted) |
Mutagen | C-BOE |
Suppressor_mutation_type | Point mutation at the orginal locus |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | 304:R>H |
A
B
C
D
E
F
A: Candidate | No selection
B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion
C: Control | No selection
D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion
E: Quantification | Illustration of the strain arrangement of the semi-quantification plate
F: Quantification | Quantification in restreaking format