Query_essential_gene_IDSPBC1773.11c
Query_essential_gene_namemug89
Query_essential_gene_productCDC50 domain protein, implicated in signal transduction (predicted)
Suppressor_gene_IDSPAC20G8.03
Suppressor_gene_nameitr2
Suppressor_gene_productMFS myo-inositol transmembrane transporter
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note471:G>E

A

B

C

D

E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format