Query_essential_gene_IDSPBC800.13
Query_essential_gene_namecnp20
Query_essential_gene_producthistone H4 variant, CENP-T ortholog
Suppressor_gene_IDSPACUNK4.16c
Suppressor_gene_nametps3
Suppressor_gene_productalpha,alpha-trehalose-phosphate synthase (predicted)
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D

E

F

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

C: Control | Selection for the OE plasmid (suppressor overexpression ON)

D: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

E: Control | Selection for the OE plasmid (suppressor overexpression OFF)

F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)