Query_essential_gene_IDSPBC800.13
Query_essential_gene_namecnp20
Query_essential_gene_producthistone H4 variant, CENP-T ortholog
Suppressor_gene_IDSPBC25B2.08
Suppressor_gene_nameSPBC25B2.08
Suppressor_gene_productSchizosaccharomyces pombe specific protein
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteTaggless ORF OE

A

B

C

D

E

F

G

H

I

J

K

L

M

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

D: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

E: Control | Selection for the OE plasmid (suppressor overexpression ON)

F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

G: Control | Selection for the OE plasmid (suppressor overexpression OFF)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

I: Quantification | Illustration of the strain arrangement of the semi-quantification plate

J: Quantification | Quantification in restreaking format (suppressor overexpression ON)_4days

K: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_4days

L: Quantification | Quantification in restreaking format (suppressor overexpression ON)_12days

M: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_12days