Query_essential_gene_IDSPBC800.13
Query_essential_gene_namecnp20
Query_essential_gene_producthistone H4 variant, CENP-T ortholog
Suppressor_gene_IDSPCC757.09c
Suppressor_gene_namernc1
Suppressor_gene_productKH domain RNA-binding protein Rnc1
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D


E

F

G

H


I

J

K

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

D: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

E: Control | Selection for the OE plasmid (suppressor overexpression ON)

F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

G: Control | Selection for the OE plasmid (suppressor overexpression OFF)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

I: Quantification | Illustration of the strain arrangement of the semi-quantification plate

J: Quantification | Quantification in restreaking format (suppressor overexpression ON)_3days

K: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_3days