Query_essential_gene_ID | SPBC800.13 |
Query_essential_gene_name | cnp20 |
Query_essential_gene_product | histone H4 variant, CENP-T ortholog |
Suppressor_gene_ID | SPCC757.09c |
Suppressor_gene_name | rnc1 |
Suppressor_gene_product | KH domain RNA-binding protein Rnc1 |
Mutagen | OP-BOE |
Suppressor_mutation_type | Overexpression-plasmid |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | C-terminally YFH-tagged ORF OE |
A
B
C
D
E
F
G
H
I
J
K
A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)
B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)
C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)
D: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)
E: Control | Selection for the OE plasmid (suppressor overexpression ON)
F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)
G: Control | Selection for the OE plasmid (suppressor overexpression OFF)
H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)
I: Quantification | Illustration of the strain arrangement of the semi-quantification plate
J: Quantification | Quantification in restreaking format (suppressor overexpression ON)_3days
K: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_3days