Query_essential_gene_IDSPBC800.13
Query_essential_gene_namecnp20
Query_essential_gene_producthistone H4 variant, CENP-T ortholog
Suppressor_gene_IDSPAC24B11.06c
Suppressor_gene_namesty1
Suppressor_gene_productMAP kinase Sty1
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteTaggless ORF OE

A

B

C

D

E

F

G

H

I

J

K

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

D: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

E: Control | Selection for the OE plasmid (suppressor overexpression ON)

F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

G: Control | Selection for the OE plasmid (suppressor overexpression OFF)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

I: Quantification | Illustration of the strain arrangement of the semi-quantification plate

J: Quantification | Quantification in restreaking format (suppressor overexpression ON)_3days

K: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_3days