Query_essential_gene_ID | SPBC1198.10c |
Query_essential_gene_name | slm5 |
Query_essential_gene_product | mitochondrial asparagine-tRNA ligase Slm5 (predicted) |
Suppressor_gene_ID | SPCC18.17c |
Suppressor_gene_name | SPCC18.17c |
Suppressor_gene_product | proteasome assembly chaperone (predicted) |
Mutagen | OP-BOE |
Suppressor_mutation_type | Overexpression-plasmid |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | C-terminally YFH-tagged ORF OE |
A
B
C
D
E
F
G
H
I
J
A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)
B: Candidate | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days"
C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)
D: Candidate | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days"
E: Control | Selection for the OE plasmid (suppressor overexpression ON)
F: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days"
G: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_10days"
H: Control | Selection for the OE plasmid (suppressor overexpression OFF)
I: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days"
J: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_10days"