Query_essential_gene_IDSPBC1198.10c
Query_essential_gene_nameslm5
Query_essential_gene_productmitochondrial asparagine-tRNA ligase Slm5 (predicted)
Suppressor_gene_IDSPCC18.17c
Suppressor_gene_nameSPCC18.17c
Suppressor_gene_productproteasome assembly chaperone (predicted)
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D


E

F

G

H

I

J

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days"

C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

D: Candidate | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days"

E: Control | Selection for the OE plasmid (suppressor overexpression ON)

F: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days"

G: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_10days"

H: Control | Selection for the OE plasmid (suppressor overexpression OFF)

I: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days"

J: Control | "Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_10days"