Query_essential_gene_ID | SPBC1198.10c |
Query_essential_gene_name | slm5 |
Query_essential_gene_product | mitochondrial asparagine-tRNA ligase Slm5 (predicted) |
Suppressor_gene_ID | SPBC19C7.03 |
Suppressor_gene_name | cyr1 |
Suppressor_gene_product | adenylate cyclase |
Mutagen | OP-BOE |
Suppressor_mutation_type | Overexpression-plasmid |
Strategy | Whether the double mutant can survive without the resucing plasmid |
Allele_note | C-terminally YFH-tagged ORF OE |
A
B
C
D
E
F
G
H
I
J
K
L
A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)
B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days
C: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_10days
D: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)
E: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days
F: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_10days
G: Control | Selection for the OE plasmid (suppressor overexpression ON)
H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_4days
I: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)_10days
J: Control | Selection for the OE plasmid (suppressor overexpression OFF)
K: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_4days
L: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)_10days