Query_essential_gene_ID | SPBC83.15 |
Query_essential_gene_name | wdr74 |
Query_essential_gene_product | ribosome biogenesis protein Nsa1 (predicted) |
Suppressor_gene_ID | SPBC1711.05 |
Suppressor_gene_name | srp40 |
Suppressor_gene_product | nucleocytoplasmic transport chaperone Srp40 (predicted) |
Mutagen | T-BOE |
Suppressor_mutation_type | Deletion |
Strategy | Whether the double mutant is viable |
Allele_note | NA |
A
B
C
D
E
F
G
A: Candidate | No selection
B: Candidate | Selection for essential gene deletion (with long-time incubation)
C: Candidate | Selection for essential and suppressor genes double mutation (with long-time incubation)
D: Candidate | Selection for essential gene deletion (replicated from plates selecting for essential gene deletion)
E: Candidate | Selection for essential and suppressor genes double mutation (replicated from plates selecting for essential and suppressor genes double mutation)
F: Quantification | Illustration of the strain arrangement of the semi-quantification plate
G: Quantification | Quantification in restreaking format