Query_essential_gene_IDSPBC83.15
Query_essential_gene_namewdr74
Query_essential_gene_productribosome biogenesis protein Nsa1 (predicted)
Suppressor_gene_IDSPBC839.05c
Suppressor_gene_namerps1701
Suppressor_gene_product40S ribosomal protein S17 (predicted)
MutagenT-BOE
Suppressor_mutation_typeDeletion
StrategyWhether the double mutant is viable
Allele_noteNA

A

B

C

D

E


F

G

A: Candidate | No selection

B: Candidate | Selection for essential gene deletion (with long-time incubation)

C: Candidate | Selection for essential and suppressor genes double mutation (with long-time incubation)

D: Candidate | Selection for essential gene deletion (replicated from plates selecting for essential gene deletion)

E: Candidate | Selection for essential and suppressor genes double mutation (replicated from plates selecting for essential and suppressor genes double mutation)

F: Quantification | Illustration of the strain arrangement of the semi-quantification plate

G: Quantification | Quantification in restreaking format