Query_essential_gene_IDSPBC725.08
Query_essential_gene_namepir2
Query_essential_gene_productzf-C2H2 type zinc finger protein, implicated in RNAi (predicted)
Suppressor_gene_IDSPBC26H8.10
Suppressor_gene_namedis3
Suppressor_gene_product3'-5' exoribonuclease subunit Dis3
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note619:Y>C

A

B

C

D

E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format