Query_essential_gene_IDSPBC839.09c
Query_essential_gene_namemrp21
Query_essential_gene_productmitochondrial ribosomal protein subunit Mrp21 (predicted)
Suppressor_gene_IDSPBP19A11.03c
Suppressor_gene_namemts4
Suppressor_gene_product19S proteasome regulatory subunit Mts4
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note412:S>F

A

B


C

D


E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format