Query_essential_gene_IDSPBC28F2.06c
Query_essential_gene_namemdm12
Query_essential_gene_productERMES complex subunit Mdm12
Suppressor_gene_IDSPAC1A6.03c
Suppressor_gene_nameSPAC1A6.03c
Suppressor_gene_productlysophospholipase (predicted)
MutagenOP-BOE
Suppressor_mutation_typeOverexpression-plasmid
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_noteC-terminally YFH-tagged ORF OE

A

B

C

D


E

F

G

H


I

J

K

L

M

A: Candidate | Selection for the OE plasmid (suppressor overexpression ON)

B: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

C: Candidate | Selection for the OE plasmid (suppressor overexpression OFF)

D: Candidate | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

E: Control | Selection for the OE plasmid (suppressor overexpression ON)

F: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression ON)

G: Control | Selection for the OE plasmid (suppressor overexpression OFF)

H: Control | Selection for essential gene deletion and the OE plasmid, and counter-selection against the rescuing plasmid (suppressor overexpression OFF)

I: Quantification | Illustration of the strain arrangement of the semi-quantification plate

J: Quantification | Quantification in restreaking format (suppressor overexpression ON)_3days

K: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_3days

L: Quantification | Quantification in restreaking format (suppressor overexpression ON)_5days

M: Quantification | Quantification in restreaking format (suppressor overexpression OFF)_5days