Query_essential_gene_IDSPBC8D2.15
Query_essential_gene_namelip5
Query_essential_gene_productmitochondrial lipoic acid synthetase Lip5 (predicted)
Suppressor_gene_IDSPAC9E9.09c
Suppressor_gene_nameatd1
Suppressor_gene_productaldehyde dehydrogenase (predicted)
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note117:I>T

A

B

C

D

E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format