Query_essential_gene_IDSPBC577.07
Query_essential_gene_nameubp10
Query_essential_gene_productubiquitin C-terminal hydrolase Ubp10 (predicted)
Suppressor_gene_IDSPAC4F8.12c
Suppressor_gene_namespp42
Suppressor_gene_productU5 snRNP complex subunit Spp42
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note571:R>H

A

B

C

D

E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format