Query_essential_gene_IDSPBC530.12c
Query_essential_gene_namepdf1
Query_essential_gene_productpalmitoyl protein thioesterase/ dolichol pyrophosphate phosphatase fusion protein Pdf1
Suppressor_gene_IDSPAC22F8.08
Suppressor_gene_namesec24
Suppressor_gene_productCOPII cargo receptor Sec24/Sfb2 subunit
MutagenC-BOE
Suppressor_mutation_typePoint mutation at the orginal locus
StrategyWhether the double mutant can survive without the resucing plasmid
Allele_note632:A>T

A

B


C

D


E

F

A: Candidate | No selection

B: Candidate | Counter-selection against the rescuing plasmid and selection for essential gene deletion

C: Control | No selection

D: Control | Counter-selection against the rescuing plasmid and selection for essential gene deletion

E: Quantification | Illustration of the strain arrangement of the semi-quantification plate

F: Quantification | Quantification in restreaking format